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Translesion synthesis catalyzed by yeast polymerase ζ. A, annealed template (28-mer)-primer (19-mer) substrate (40 nm) was incubated with polymerase ζ (35.2 nm) in the presence (+) or absence (–) of REV1 (35.7 nm) in a 10-μl reaction mixture at 37 °C for 60 min. Asterisks indicate 19-mer (*), 20-mer (**), and 28-mer (***). B, nucleotide insertion opposite template dC located 5′ to H-εdC. Reaction was conducted at 37 °C for 60 min in the presence of polymerase ζ (35.2 nm).
