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In vitro phosphorylation of Nedd4-2 by IKKβ. Phosphorylation of Nedd4-2 (left) or GST-IκBα (right) in the absence (–) or presence (+) of purified SGK1 or IKKβ, as indicated, is shown. Phospho-screen (upper panel) and Nedd4-2 immunoblot (lower panel) images from the same nitrocellulose membrane are shown. Autophosphorylation of IKKβ is also indicated on the phospho-screen. Images shown are representative of those obtained in three replicate experiments.
