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. 2009 Jan 2;284(1):685–695. doi: 10.1074/jbc.M806124200

FIGURE 4.

FIGURE 4.

ASK1, MKK4, and JNK3 interact with both domains of arrestin. A-C, HEK-293A cells were transfected with HA-ASK-NLS or GFP-JNK3 individually or with FLAG-tagged full-length arrestin3 (FLAG-Arr3), its N- (FLAG-Arr3-N) or C-domain (FLAG-Arr3-C), arrestin2-NES+ (Arr2-NES), arrestin2 N-domain-NES (Arr2-N-NES), or arrestin2 C-domain-NES (Arr2-C-NES). Immunocytochemistry was done as described in Fig. 2C. GFP-JNK3 was visualized by its intrinsic fluorescence. Representative images are shown. The localization of GFP-JNK and HA-ASK1-NLS was quantified, as described in the legend to in Fig. 2C. Means ± S.D. (n = 3) of the fraction of cells with more JNK3 or ASK1 in the cytoplasm than in the nucleus also shown. (****, p < 0.0001). D-F, COS-7 cells were transfected with HA-ASK1, HA-MKK4, or GFP-JNK3 alone, or together with FLAG-tagged arrestin3 and its domains. Immunoprecipitation and Western blot analysis were performed as described in the legend to Fig. 2A.