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. 2009 Jan 2;284(1):685–695. doi: 10.1074/jbc.M806124200

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Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Kinase and C-domains are the key arrestin-binding elements in ASK1. A, Constructs containing different domains of ASK1: N domain (ASK1-N), N and Kinase domains (ASK1-(N+K)), C domain (ASK1-C), C and kinase domains (ASK1-(C+K)). B and C, COS-7 cells were transfected with HA-tagged ASK1 constructs shown in panel A, together with FLAG-arrestin3 (FLAG-Arr3) (panel B), or HA-ASK1-(C+K) with full-length arrestin3 (Arr3) or its N- (Arr3-N) and C-domain (Arr3-C) (panel C), respectively. Immunoprecipitation and Western blot analysis were performed as described in the legend to Fig. 2A.