TABLE 2.
Activity of RNase R-truncated proteins on single-stranded RNA Activity assays were performed as described under “Experimental Procedures.” Each value represents the mean of at least three experiments.
|
Substrate
|
Activitya
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Full-length | ΔBasic | ΔCSDs | ΔS1 | ΔCSDsΔS1 | |||||
| nmol min–1 nmol–1 | |||||||||
| Poly(A) | 460 ± 27 | 980 ± 84 | 330 ± 39 | 12 ± 3.0 | 2.0 ± 1.0 | ||||
| A17 | 33 ± 9.9 | 95 ± 36 | 2.8 ± 1.8 | 0.7 ± 0.1 | 0.007 ± 0.002 | ||||
| A4 | 0.3 ± 0.2 | 0.6 ± 0.1 | 0.2 ± 0.1 | 0.1 ± 0.01 | 0.2 ± 0.1 | ||||
a
For poly(A) this value represents nmol of AMP released per min per nmol of RNase R. For A17 and A4 this value represents nmol of substrate oligoribonucleotide degraded per min per nmol RNase R