TABLE 3.
Activity of truncated RNase R proteins on a substrate containing a duplex Activity assays were performed as described under “Experimental Procedures.” Each value represents the mean of at least three experiments.
|
Substrate
|
Activitya
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Full-length | ΔBasic | ΔCSDs | ΔS1 | ΔCSDsΔS1 | |||||
| nmol min–1 nmol–1 | |||||||||
| ss17-A17 | 5.5 ± 2.6 | 11 ± 0.7 | 0.5 ± 0.1 | 0.6 ± 0.1 | 0.005 ± 0.0002 | ||||
| ds17-A17 | 0.5 ± 0.2 | 0.7 ± 0.4 | 0.7 ± 0.2 | 0.02 ± 0.01 | 0.003 ± 0.0009 | ||||
a
This value represents nmol of substrate oligoribonucleotide degraded per min per nmol of RNase R