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A, separation of small intestine into crypts, villi, and mesenchyme and muscle fractions and confirmation of purity by Western blot analysis of putative markers for each fraction. B, Western blot demonstrated efficient deletion of pRb from crypts and villi in VC+Rb(f/f) mice. The pRb protein from isolated crypts revealed hyperphosphorylation. Actin was used as a loading control. C, crypts; V, villi.
