FIGURE 8.

CTD is sufficient for oligomerization. VARD and CTD were separated on a 15% acrylamide Laemmli gel and transferred to nitrocellulose for immunodetection. The samples were loaded with or without 20 mm dithiothreitol, as indicated. COMMD1 monomer, dimer, and tetramer sizes are labeled with the letters “m,” “d,” and “t”(left), respectively. CTD species are indicated with as “mCTD,” “dCTD,” and “tCTD”(right). B, redox regulation of COMMD1 oligomerization with indicated treatments.