FIGURE 3.
Reciprocal relative rate versus relative viscosity plots of wild-type β-LS and K443R at 25 °C. The viscosity dependence on kcat and kcat/Km is shown in A and B, respectively. The dashed line in B corresponds to a slope of 0. Microviscogens glycerol (0–35% (w/v)) and ethylene glycol (0–35% (w/v)) are shown as • and ▪ for wild-type β-LS, respectively. The control macroviscogen, PEG 8000 (0–6% (w/v)), is denoted as □ and × for wild-type β-LS and K443R, respectively. ○ represents the dependence of K443R on glycerol (0–30% (w/v)). Error bars are shown for all data points and do not exceed the size of the data points when not visible.