FIGURE 4.
Kinetic results demonstrate cooperative behavior for S. enterica ApbC protein when ATP is varied (wild-type ApbC shown). The plots illustrate the velocity of ADP production while varying their concentration of ATP. Plots are as follows: A, v versus [substrate] fit by Equation 3; B, double-reciprocal plot of the data in plot 1 fit by Equation 4; C, Hill plot fit using a linear regression (n = slope). Datum points are the average of three assays. v is defined as nmol/min/mg.