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Time and protein concentration dependence of R. leguminosarum LpxE. Dephosphorylation of [4′-32P]lipid IVA is linear with time (panel A) and protein concentration when assayed for 10 min under standard conditions at pH 6.5 (panel B). After prolonged incubation, or in the presence of high enzyme concentrations, the reaction goes to completion (data not shown). The data shown in each panel are from a single representative experiment.
