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Steady state kinetics of purified LpxE in a mixed micelle system. Standard assay conditions were used, but the lipid IVA substrate concentrations were varied. A fit of the Michaelis-Menten equation to the data gives an apparent Km of 11 ± 2 μm for lipid IVA and an apparent Vmax of 3.3 ± 0.2 nmol/min/mg. The data shown are the average of several experiments with the standard deviation shown.
