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Time course of inactivation of R. leguminosarum LpxE by DEPC. Purified LpxE (20 μm) was preincubated at room temperature with 0 (○), 10 μm (▾), 20 μm (♦), 40 μm (▪), or 80 μm (•) DEPC. At the indicated times, a portion of the preincubation mixture was quenched with imidazole, diluted, and assayed for remaining LpxE activity. The points are connected for ease of visualization. The data shown are from a single representative experiment.
