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Relative protein abundance of NR salvage enzymes. Yeast strains, containing tandem affinity purification-tagged NR salvage enzymes, were grown in synthetic media without or with the addition of 100μm NR. The relative abundance of the proteins, Meu1 > Urh1 > Nrk1 > Pnp1, was determined by Western blotting with an anti-tandem affinity purification antibody CAB1001 (Open Biosystems). Half of the blot was probed with anti-actin antibody as a loading control.
