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. 2009 Jan 2;284(1):158–164. doi: 10.1074/jbc.M807976200

TABLE 1.

Kinetic parameters of Pnp1, bovine Pnp and Urh1

Km kcat kcat/Km
μm s-1 s-1m-1
Pnp1
    Inosine 33.0 ± 5.2 3.8 ± 0.1 115,000
    NR 1,020 ± 140 11. ± 0.6 11,000
    NaR 3,000 ± 1,000 0.13 ± 0.02 40
Bovine Pnp
    Inosine 51.0 ± 5.1 14 ± 3.8 275,000
    NR 900 ± 100 31.2 ± 2.2 35,000
    NaR 900 ± 230 4.4 ± 0.6 4,900
Urh1
    Uridine 1,600 ± 500 20.0 ± 4.4 125,000
    NR 16.0 ± 2 23.4 ± 0.8 1,430,000
    NaR 150.0 ± 40 1.8 ± 0.9 12,000
    MeNaR 45.0 ± 15 2.3 ± 0.2 51,000