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pKa values determined for the alkaline transition in control and nitro-Tyr-97- and nitro-Tyr-74-cytochrome c by NMR and optical spectroscopy
The data were fitted to the Henderson-Hasselbalch function to obtain the pKa values. NMR determinations were performed at 318 K and optical determinations at 298 K.
|
Sample
|
pKa values
|
|
|---|---|---|
| NMR | 695 nm band | |
| Control | 9.3 ± 0.1 | 9.4 ± 0.1 |
| Nitro-Tyr-97 | 8.6 ± 0.2 | 8.7 ± 0.2 |
| Nitro-Tyr-74 | 7.2 ± 0.1 | 7.3 ± 0.1 |
