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Effect of S-glutathionylation on GST enzymatic activity The values represent the mean of 3 independent experiments ± S.D.
| GST specific activity | |
|---|---|
| μmol/mg protein/min | |
| Cells | |
| HEK-VA | 59.3 ± 3.2 |
| HEK-WT | 75.3 ± 2.1a |
| HEK-Y7F | 12.4 ± 5.1a |
| HEK-C47A | 12.8 ± 1.8a |
| HEK-C101A | 13.8 ± 0.8a |
| Purified protein | |
| GST | 53.2 ± 1.6 |
| GST + NOV-002 | 49.1 ± 2.3 |
| GST + PABA/NO | 13.0 ± 0.9a |
| GST(Y7F) | 4.5 ± 0.1a |
| GST(C47A) | 5.3 ± 2.5a |
| GST(C101A) | 10.1 ± 0.9a |
p < 0.01
