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Amplex Red probe reacts with organic/fatty acid hydroperoxides besides hydrogen peroxide. Fig. 3 shows the dose response (0–2,500 pmol) when hydrogen peroxide (A) and the hydroperoxides, t-BHP (open triangle) and 15HpETE (closed triangle) were incubated with the Amplex Red probe (B), and end point measurements were made. Both 15HpETE and t-BHP increased the Amplex Red signal in a dose-dependent manner but at levels much lower than with H2O2.
