FIGURE 5.

A, cPLA₂ inhibitor inhibits the Amplex Red signal in mitochondria from denervated muscle. The Amplex Red assay was performed in the absence/presence of the cPLA₂ inhibitor, AACOCF₃ (20 μm) with substrates (glutamate/malate (G/M), 5 mm; succinate (S), 10 mm) and inhibitors (rotenone (R), antimycin A (AA), 0.5 μm). AACOCF₃ inhibited the Amplex Red signal in mitochondria from denervated muscle to control values in the absence (State 1) and in the presence of respiratory substrates (glutamate/malate and succinate/rotenone). The values are the means ± S.E. for six or seven experiments. Statistical significance was based on the difference in Amplex Red signal in the absence (–) and presence (+) of AACOCF₃ within each experimental condition (*, p < 0.001; **, p < 0.01) by one-way ANOVA with Newman Keul's multiple comparison test. (control, white bars; denervated (–AACOCF₃), black bars; denervated (+AACOCF₃), gray bars). B, cPLA₂ inhibitor inhibits the Amplex Red signal in mitochondria from Sod1^–/– mice. The Amplex Red assay was performed in the presence/absence of AACOCF₃ (20μm) in 12-month-old Sod1^–/– mice. AACOCF₃ inhibited the Amplex Red signal (State 1) to control values. The values are the means ± S.E. for three or four experiments. Statistical significance was based on the difference in Amplex Red signal in the absence (–) and presence (+) of AACOCF₃ in Sod1^–/– (*, p < 0.001) by one-way ANOVA with Newman Keul's multiple comparison test (wild type, white bars; Sod1^–/–, black bars).