FIGURE 8.

Inhibition of muscle atrophy and decrease in Amplex Red signal in Gpx4-Tg mice in response to denervation. Denervation was induced by surgical transection of the sciatic nerve at the level of femur. The contralateral limb served as a control. The mice were sacrificed, tissues were collected, and Amplex Red signal was measured 7 days after surgery in mitochondria isolated from control and denervated muscle from WT and Gpx4-Tg mice (State 1). A, loss of gastrocnemius muscle mass/100 g of body weight in WT and Gpx4Tg mice in response to denervation. B, denervated/control muscle ratio in WT and Gpx4-Tg mice (*, p = 0.0001) by Student's t test. C, Amplex Red signal in mitochondria from WT and Gpx4-Tg mice in response to denervation surgery. Statistical significance was based on the difference in Amplex Red signal in mitochondria from WT and Gpx4-Tg denervated muscle (*, p < 0.05) by one-way ANOVA with Newman Keul's multiple comparison test. The values are expressed as the means ± S.E. of n = 8 mice. A and C, control, white bars; denervated, black bars. B, WT, white bar; Gpx4-Tg, black bar.