TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description of strain or plasmid | Source |
|---|---|---|
| E. coli | ||
| S17.1 | E. coli shuttle vector. 294(recA, pro res mod+) Tpr, Smr, (pRP4-2 Tc::Mu-Km::Tn7) | (61) |
| P. aeruginosa | ||
| MPAO1 | Wild-type P. aeruginosa PAO1 | Pseudomonas Genome Center, Seattle, WA |
| 7520 | phuS mutant containing ISlacZ/hah transposon insertion, derived from MAPO1 | (as above) |
| CDC-5 | pvd-2 derivative of PAO1 strain lacking pyoverdin production | (35) |
| IA614 | Pyochelin-deficient derivative of CDC-5 strain, obtained by ethylmethanesulfonate mutagenesis | (35) |
| IR1648 | Chromosomal knockout of iron-regulated heme oxygenase gene, hemO derived from IA614 | (46) |
| IA614-ΔphuS | Chromosomal knockout of phuS derived from IA614 | This study |
| IR1648-ΔphuS | Chromosomal knockout of phuS derived from IR1648. This mutant lacks both pigA and phuS along with the siderophores pyochelin and pyoverdin | This study |
| Plasmids | ||
| pEX18p | AmpR; allelic replacement vector | (34) |
| pFlp2 | AmpR; source of Flp recombinase | (34) |
| pPS856 | Source of GentR-GFP, GentR-conferring fragment flanked by FRT sites | (34) |
| pFTC1 | Source of TetR-GFP, TetR-conferring fragment flanked by FRT sites | H. Schweizer |
| pEX18p-ΔphuS::Gm | AmpR; allelic replacement vector containing 1.8-kb fragment of in-frame phuS deletion containing the GentR antibiotic marker | This study |
| pEX18p-ΔphuS::tet | AmpR; allelic replacement vector containing 1.8-kb fragment of in-frame phuS deletion containing the TetR antibiotic marker | This study |