TABLE 2.

Differential transcription of the PQS and pyocyanin biosynthesis and efflux genes

O.D., optical density at 600 nm.

Open reading frame	Gene name	Product name	IA614aversus			MPAO1bversus
			IA614-ΔphuS	IR1648 (hemO)	IR1648-(hemO/ΔphuS)	7520 phuS::Tn	7520 phuS::Tn
				O.D. 1.5		O.D. 1.5	O.D. 3.0
PQS biosynthesis genes
PA0996	pqsA	Probable coenzyme A ligase	4.2	NSc	NS	6.4	10.2
PA0997	pqsB	Homologous to β-keto-acyl-acyl carrier protein synthase	7.8	NS	1.2	12.1	5.3
PA0998	pqsC	Homologous to β-keto-acyl-acyl carrier protein synthase	10.0	NS	NS	16.2	5.3
PA0999	pqsD	3-Oxoacyl-[acyl-carrier-protein] synthase III	6.5	NS	NS	10.4	5.2
PA1000	pqsE	Quinolone signal response protein	3.0	NS	NS	6.0	7.0
PA1001	phnA	Anthranilate synthase component I	3.4	NS	NS	5.8	10.0
PA1002	phnB	Anthranilate synthase component II	1.7	NS	NS	3.0	5.4
PA1003	pqsR	Transcriptional regulator MvfR	1.2	NS	NS	NS	2.0
Phenazine biosynthesis genes
PA1898	qscR; phzR	Quorum-sensing control repressor	NS	NS	NS	NS	2.7
PA1901	phzC2	Phenazine biosynthesis protein PhzC	NS	NS	1.2	1.20	15.4
PA1902	phzD2	Phenazine biosynthesis protein PhzD	NS	-1.20	NS	1.30	13.9
PA1903	phzE2	Phenazine biosynthesis protein PhzE	NS	NS	NS	1.32	18.2
PA1904	phzF2	Probable phenazine biosynthesis protein	NS	NS	NS	NS	17.9
PA1905	phzG2	Probable pyridoxamine 5′-phosphate oxidase	1.1	NS	NS	1.5	23.0
PA4209	phzM	Probable phenazine-specific methyltransferase	NS	NS	NS	1.3	7.8
PA4210	phzA1	Probable phenazine biosynthesis protein	NS	NS	NS	NS	40.32
PA4211	phzB1	Probable phenazine biosynthesis protein	1.3	NS	NS	1.8	34.0
PA4217	phzS	Flavin-containing monooxygenase	NS	-1.2	-1.1	1.5	12.7
Pyocyanin regulated efflux pumps
PA4205	mexG	Hypothetical protein	2.6	NS	NS	NS	81.9
PA4206	mexH	Probable RND efflux membrane fusion	2.5	NS	NS	NS	26.6
PA4207	mexI	Probable RND efflux transporter	1.8	NS	NS	NS	12.1
PA4208	opmD	Probable outer membrane protein precursor	1.7	NS	NS	NS	5.8

^aRatio of expression of the IA614-ΔphuS, IR1648, and IR1648-ΔphuS deletion strains compared to IA614 from three separate microarray experiments. Changes were considered significant at or below a 0.05 FDR as described under “Experimental Procedures.”

^bRatio of expression of the 7520 phuS::Tn strain compared to MPA01 from three separate microarray experiments. Changes were considered significant at or below a 0.05 FDR as described under “Experimental Procedures.”

^cNS, not significant.