TABLE 3.

Differential transcription of the pyoverdin and pyochelin biosynthesis and uptake genes

O.D., optical density at 600 nm.

Open reading frame	Gene name	Product name	IA614aversus			MPAO1bversus
			IA614-ΔphuS	IR1648 (hemO)	IR1648-ΔphuS	7520 phuS::Tn	7520 phuS::Tn
				O.D. 1.5		O.D. 1.5	O.D. 3.0
Pyoverdin biosynthesis and uptake system
PA2383		Probable transcriptional regulator	-1.2	NSc	-1.2	NS	2.3
PA2384		Hypothetical protein	1.2	NS	NS	-1.3	11.2
PA2385	pvdQ	PvdQ	NS	NS	NS	NS	2.4
PA2386	pvdA	l-ornithine N5-oxygenase	NS	NS	1.30	-1.4	NS
PA2392	pvdP	PvdP	NS	NS	NS	NS	2.3
PA2393		Probable dipeptidase precursor	NS	NS	NS	NS	4.17
PA2394	pvdN	PvdN	NS	-1.1	1.3	NS	2.9
PA2396	pvdF	Pyoverdine synthetase F	NS	NS	NS	NS	3.2
PA2397	pvdE	Pyoverdine biosynthesis protein PvdE	NS	NS	NS	NS	2.8
PA2398	fpvA	Ferripyoverdine receptor	-1.2	NS	NS	-2.9	2.4
PA2399	pvdD	Pyoverdine synthetase D	NS	NS	1.4	NS	2.4
PA2413	pvdH	l-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase	NS	NS	1.3	NS	3.8
PA2425	pvdG	PvdG	NS	NS	NS	NS	2.6
PA2426	pvdS	Sigma factor PvdS	1.1	NS	NS	-1.3	13.5
Pyochelin biosynthesis and uptake genes
PA4218	fptX	Probable transporter	NS	NS	1.4	7.4	1.4
PA4219	fptC; yfpB	Hypothetical protein	NS	NS	1.3	3.3	NS
PA4220	fptB	Hypothetical protein	NS	NS	1.5	13.4	2.0
PA4221	fptA	Fe(III)-pyochelin outer membrane receptor precursor	NS	NS	1.3	4.7	NS
PA4222	pchI	Probable ATP-binding component of ABC transporter	NS	NS	1.2	4.1	NS
PA4223	pchH	Probable ATP-binding component of ABC transporter	NS	NS	1.2	6.2	NS
PA4224	pchG	Pyochelin biosynthetic protein PchG	NS	NS	NS	5.5	NS
PA4225	pchF	Pyochelin synthetase	NS	NS	1.2	5.1	NS
PA4226	pchE	Dihydroaeruginoic acid synthetase	NS	NS	NS	4.7	NS
PA4227	pchR	Transcriptional regulator PchR	-1.1	NS	NS	NS	4.0
PA4228	pchD	Pyochelin biosynthesis protein PchD	NS	-1.2	1.2	2.6	NS
PA4229	pchC	Pyochelin biosynthetic protein PchC	NS	NS	1.4	2.7	NS
PA4230	pchB	Salicylate biosynthesis protein PchB	NS	NS	1.2	6.5	NS
PA4231	pchA	Salicylate biosynthesis isochorismate synthase	NS	NS	1.3	4.5	NS

^aRatio of expression of the IA614-ΔphuS, IR1648, and IR1648-ΔphuS deletion strains compared to IA614 from three separate microarray experiments. Changes were considered significant at or below a 0.05 FDR as described under “Experimental Procedures.”

^bRatio of expression of the 7520 phuS::Tn strain compared to MPA01 from three separate microarray experiments. Changes were considered significant at or below a 0.05 FDR as described under “Experimental Procedures.”

^cNS, not significant.