TABLE 4.

Differential transcription of iron-regulated genes involved in iron acquisition and metabolism

O.D., optical density at 600 nm.

Open reading frame	Gene name	Product name	IA614aversus			MPAO1bversus
			IA614-ΔphuS	IR1648 (hemO)	IR1648-ΔphuS	7520 phuS::Tn	7520 phuS::Tn
			O.D. 1.5					O.D. 1.5	O.D. 3.0
PA0470	fiuA	Ferrichrome receptor FiuA	-1.2	-1.2	NSc	NS	NS
PA0471	fiuR	Probable transmembrane sensor	1.2	NS	1.2	-1.8	1.7
PA0472	fiuI	Probable sigma-70 factor, ECF subfamily	1.2	NS	1.3	-1.9	2.5
PA0672	hemO	Heme oxygenase	NS	NS	1.2	-1.6	2.4
PA0693	exbB2	Transport protein ExbB2	4.8	NS	NS	NS	1.8
PA0694	exbD2	Transport protein ExbD	2.59	NS	NS	NS	NS
PA0929	pirR	Two-component response regulator	1.2	-1.2	NS	-2.6	2.1
PA1302	hxuC	Probable heme utilization protein precursor	NS	NS	1.3	NS	NS
PA1365		Probable siderophore receptor	NS	NS	NS	NS	-1.3
PA2686	pfeR	Two-component response regulator PfeR	NS	NS	NS	NS	1.3
PA3397	fpr	Ferredoxin-NADP+ reductase	NS	NS	-1.1	NS	2.8
PA3410		Probable sigma-70 factor, ECF subfamily	NS	NS	1.2	-1.3	2.7
PA3530	bfd	Conserved hypothetical protein	1.1	NS	1.2	-1.3	23.5
PA3531	bfrB	Bacterioferritin	-1.3	1.6	-1.1	1.5	-6.5
PA3812	iscA	Probable iron-binding protein IscA	NS	NS	NS	NS	1.6
PA3814	iscS	l-cysteine desulfurase	NS	NS	NS	-1.3	3.0
PA3899		Probable sigma-70 factor, ECF subfamily	NS	NS	1.2	-1.3	1.6
PA3901	fecA	Fe(III) dicitrate transport protein FecA	-1.5	NS	-1.2	-1.5	-2.5
PA4359	feoA	Conserved hypothetical protein	1.9	NS	-1.2	-2.2	8.7
PA4513	piuB	Probable oxidoreductase	NS	NS	1.13	-1.1	NS
PA4514	piuA	Probable outer membrane iron-receptor	-1.4	NS	1.4	-2.0	NS
PA4515	piuC	Conserved hypothetical protein	1.2	-1.3	1.2	-3.4	4.4
PA4675	optH; iutA	Probable TonB-dependent receptor	-2.0	NS	NS	-2.2	-2.4
PA4687	hitA	Ferric iron-binding periplasmic protein HitA	NS	-1.4	-1.3	-2.4	NS
PA4688	hitB	Iron (III)-transport system permease HitB	-1.2	NS	-1.1	-1.8	NS
PA4706	phuV	ABC transporter (ATPase)	1.2	NS	1.5	8.6	3.4
PA4708	phuT	Heme-transport protein, PhuT	1.8	NS	1.9	13.2	10.8
PA4709	phuS	Probable hemin degrading factor	NS	NS	NS	NS	1.8
PA4710	phuR	Heme/hemoglobin uptake outer membrane receptor PhuR precursor	2.4	NS	2.5	1.7	2.3
PA4896		Probable sigma-70 factor, ECF subfamily	NS	-1.2	NS	-1.4	2.1
PA5217		Probable component of ABC iron transporter	1.2	NS	1.2	-3.4	NS
PA5531	tonB	TonB protein	1.3	-1.3	1.2	-3.4	4.2

^aRatio of expression of the IA614-ΔphuS, IR1648, and IR1648-ΔphuS deletion strains compared to IA614 from three separate microarray experiments. Changes were considered significant at or below a 0.05 FDR as described under “Experimental Procedures.”

^bRatio of expression of the 7520 phuS::Tn strain compared to MPA01 from three separate microarray experiments. Changes were considered significant at or below a 0.05 FDR as described under “Experimental Procedures.”

^cNS, not significant.