Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Dec 12;1:2. doi: 10.3389/neuro.16.002.2008

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2008 Lee, Malarkey, Reyes and Parpura.

This is an open-access article subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.

PMC Copyright notice

Micropatterned coverslip preparation scheme. (A) A polyethyleneimine (PEI)-coated coverslip was placed on top the PDMS mold with the PEI-coated side facing the PDMS mold as shown in (B). (C) Boiling agarose (2% w/v in water) was added to one of the open ends while gentle suction was applied at the other end to draw agarose into the channel until it was completely filled. The agarose was allowed to cool down and then the micropatterned coverslip was removed from the PDMS mold. (D) The micropatterned coverslip consists of a micropit array in the middle portion with non-pit segmental regions on each side. Plating and culturing astrocytes on these micropatterned coverslips yields cells grown in the micropit regions as shown in the schematic diagram in (E) as well as grouped cells in the two non-micropit regions on either side.