Table 2.
Mean (±SEM) ethanol preference (E:T), water intake (ml), total fluid intake (ml), food intake (g/kg body weight), and body weight for rats in each of the ethanol history groups during the 4 hours and 24 hours following infusion with one of four NPY doses (0.0, 2.5, 5.0, or 10.0 µg). Analyses of data from VAPOR ABST and CHOICE ABST rats revealed that 5.0 µg & 2.5 µg NPY suppressed 4-hr ethanol preference (p<0.05) and increased 4-hr water intake (p<0.001) relative to aCSF controls and also increased 4-hr total fluid intake in VAPOR ABST rats (p<0.001) relative to aCSF controls; all doses of NPY increased food intake (p<0.05) at 4 hrs and 24 hrs post-infusion; and VAPOR ABST rats weighed less than CHOICE ABST rats on infusion day (p<0.05). Analyses of data from VAPOR ABST and VAPOR CONT rats revealed that all NPY doses suppressed 4-hr ethanol preference (p<0.001), increased 4-hr water intake (p<0.05), increased 4-hr fluid intake (p<0.05), and increased food intake at 4 and 24 hours (p<0.05) post-infusion relative to aCSF controls; the 5.0 µg & 2.5 µg NPY doses increased 24-hr water intake (p<0.05) and 24-hr total fluid intake (p<0.05) relative to aCSF controls; and VAPOR ABST rats weighed more than VAPOR CONT rats on infusion day (p<0.001).
| Ethanol History | Time Post-Inf. | NPY Dose | Ethanol Preference | Water Intake (ml) | Food Intake (g/kg) | Total Fluid Intake (ml) | Body Weight (g) |
|---|---|---|---|---|---|---|---|
| VAPOR | 4hr | aCSF | 0.25 (0.03) | 10.51 (1.14) | 17.41 (1.33) | 13.63 (1.12) | 301.18 (7.67) |
| ABST | 2.5 µg | 0.17 (0.03) | 17.79 (1.14) | 29.52 (2.03) | 21.17 (0.99) | 329.53 (9.08) | |
| 5.0 µg | 0.12 (0.01) | 21.05 (1.04) | 40.19 (1.91) | 23.85 (0.96) | 324.41 (7.99) | ||
| 10.0 µg | 0.18 (0.03) | 13.66 (1.65) | 33.59 (2.88) | 16.29 (1.77) | 317.11 (5.69) | ||
| 24 hr | aCSF | 0.19 (0.04) | 28.52 (2.49) | 53.76 (3.93) | 34.71 (2.06) | ||
| 2.5 µg | 0.18 (0.03) | 36.10 (2.50) | 66.87 (2.52) | 43.78 (2.17) | |||
| 5.0 µg | 0.11 (0.01) | 38.09 (2.22) | 70.88 (3.06) | 42.64 (2.10) | |||
| 10.0 µg | 0.18 (0.03) | 28.81 (3.55) | 65.59 (5.84) | 33.78 (3.72) | |||
| CHOICE | 4 hr | aCSF | 0.22 (0.03) | 14.09 (1.59) | 18.23 (1.26) | 17.71 (1.62) | 340.13 (10.77) |
| ABST | 2.5 µg | 0.15 (0.01) | 18.44 (1.29) | 31.10 (1.88) | 21.71 (1.42) | 331.00 (11.60) | |
| 5.0 µg | 0.15 (0.02) | 17.74 (1.34) | 33.67 (3.20) | 20.55 (1.21) | 325.33 (6.05) | ||
| 10.0 µg | 0.17 (0.02) | 12.78 (1.33) | 30.11 (2.09) | 14.99 (1.36) | 326.38 (9.15) | ||
| 24 hr | aCSF | 0.26 (0.05) | 31.94 (2.66) | 51.05 (2.45) | 39.36 (2.59) | ||
| 2.5 µg | 0.26 (0.04) | 35.04 (1.91) | 65.44 (2.58) | 42.44 (2.51) | |||
| 5.0 µg | 0.20 (0.04) | 35.66 (3.48) | 64.45 (4.03) | 42.49 (2.94) | |||
| 10.0 µg | 0.18 (0.04) | 27.99 (2.92) | 61.06 (4.66) | 32.46 (3.10) | |||
| VAPOR | 4 hr | aCSF | 0.34 (0.06) | 10.69 (1.74) | 14.70 (1.61) | 14.48 (1.91) | 312.00 (8.38) |
| CONT | 2.5 µg | 0.17 (0.02) | 16.64 (1.42) | 22.17 (1.98) | 19.83 (1.38) | 307.84 (8.66) | |
| 5.0 µg | 0.19 (0.04) | 20.00 (1.86) | 32.35 (2.99) | 23.84 (1.62) | 286.05 (7.55) | ||
| 10.0 µg | 0.19 (0.03) | 16.94 (1.59) | 32.64 (2.58) | 20.34 (1.78) | 291.13 (7.34) | ||
| 24 hr | aCSF | 0.23 (0.03) | 29.91 (3.52) | 40.50 (4.76) | 38.49 (4.17) | ||
| 2.5 µg | 0.17 (0.02) | 40.43 (3.85) | 53.08 (4.50) | 47.85 (3.91) | |||
| 5.0 µg | 0.18 (0.04) | 43.73 (3.73) | 65.59 (4.78) | 52.38 (3.13) | |||
| 10.0 µg | 0.16 (0.02) | 39.52 (4.00) | 64.29 (6.01) | 47.33 (4.93) | |||