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. Author manuscript; available in PMC: 2009 Jul 25.
Published in final edited form as: Cell. 2008 Jul 25;134(2):231–243. doi: 10.1016/j.cell.2008.06.035

Figure 5. Chromatin reassembly is not required for removal of Mec1-Ddc2 or phosphorylated H2A after DNA repair, but is required for checkpoint adaptation.

Figure 5

A. Removal of Ddc2 from the site of DNA repair does not require chromatin assembly. The HO lesion was induced in strains WT (JFY016) and asf1Δ (JFY017) at time 0 by addition of galactose. The level of Ddc2 flanking the HO lesion during SSA repair was measured by ChIP analysis. B. Loss of phosphorylated H2A from chromatin does not require Asf1. The HO lesion was induced in strains WT (YMV045), asf1Δ (JKT200), and rad52Δ (YMV046) at time 0 by addition of galactose. The level of H2A phosphorylated on serine 129 flanking the HO site in strains undergoing HO repair by SSA was measured by ChIP analysis. C. Asf1 contributes to checkpoint adaptation. Colony formation was assessed at the indicated times after placing single unbudded cells onto galactose plates to induce the unrepairable DSB in isogenic WT, kuΔ and asf1Δ strains derived from JKM179.