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. Author manuscript; available in PMC: 2009 Nov 16.
Published in final edited form as: Immunol Lett. 2008 Sep 21;121(1):74–83. doi: 10.1016/j.imlet.2008.08.004

Figure 7. Apigenin suppresses another NF-κB-regulated anti-apoptotic and inflammatory gene product in T cells.

Figure 7

Rested short-term line CD4 T cells (2 × 106 cells/well) were treated with various concentrations of apigenin for 2 hours before being exposed to plate-coated anti-CD3 plus anti-CD28 for the indicated times. Cytoplasmic extracts were then prepared, fractionated with SDS-PAGE, and electrotransferred to a PVDF membrane, after which a western blot analysis was performed with anti-COX2 polyclonal antibody. Apigenin suppresses COX-2 protein expression in a dose- and time-dependent manner. The results are representative of three independent experiments.