Table 2.
Effect of PA, EA, POMx and PJ on gene expression of HSD3B2, AKR1C3, SRD5A1 and AR in LNCaP, LNCaP-AR and DU-145 prostate cancer cells
| Cell Line/Treatment | HSD3B2 | AKR1C | SRD5A1 | AR |
|---|---|---|---|---|
| LNCaP | ||||
| PA | NS | ND | NS | NS |
| EA | NS | ND | NS | NS |
| POMx | ↓ | ND | NS | ↓ |
| PJ | NS | ND | NS | NS |
| LNCaP AR | ||||
| PA | ↓ | ND | ↓ | ↓ |
| EA | ↓ | ND | ↓ | NS |
| POMx | ↓ | ND | ↓ | ↓ |
| PJ | ↓ | ND | ↓ | ↓ |
| DU-145 | ||||
| PA | ND | NS | NS | ND |
| EA | ND | ↓ | NS | ND |
| POMx | ND | NS | ↓ | ND |
| PJ | ND | NS | NS | ND |
Cells were incubated with punicalagin (PA 50 μg/ml)), ellagic acid (EA, 50 μg/ml), pomegranate extract (POMx, 50 μg/ml) or pomegranate juice (PJ, 100 μg/ml) for 48 h. n=4-6. ↓: More than two fold reduction at P <.05. NS: not significantly different compared to control (0.1% DMSO) at P <.05. ND: Not detected indicates very low levels of gene expression in these cells.