phoP mutations increase HmsT expression in Y. pseudotuberculosis. Total protein from strains in the YPIII (A) and IP2666c (B) backgrounds was immunoblotted with a rabbit polyclonal antibody against HmsT. The antibody is known to cross-react with multiple other proteins (Perry, et al., 2004); the HmsT band (arrow) is identified by its absence in the hmsT mutant. 1, YPIII; 2, CDY103; 3, YPIII/MMB67EH; 4, YPIII/pPhoPKIM; 5, IP2666c; 6, IP266cphoPΔ.