Figure 3. Loss of FleQ or FleN does not cause elevated c-di-GMP levels.
C-di-GMP was extracted from whole cells and levels were measured as described in the Experimental Procedures. Strains are wild type (PAO1), a fleQ mutant, a wspF mutant, a fleQ wspF mutant, a fleN mutant, and a fleN wspF mutant. Data represent averages of three independent cultures. Error bars are the standard deviations between independent cultures.