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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Exp Eye Res. 2008 May 28;87(3):176–183. doi: 10.1016/j.exer.2008.05.010

Figure 1. Expression of Kir channel subunits in native human RPE and neural retina.

Figure 1

Total RNAs isolated from pooled human RPE sheets and neural retina from five eye donors were treated with DNase I, then reverse transcribed with random decamers. RT reactions were performed in the presence (+) or absence (−) of reverse transcriptase. PCR was performed using a primer set specific for each Kir subunit or GAPDH (as endogenous control). The identities of all Kir products from the RPE were confirmed by DNA sequencing.