Figure 2.

'Results' in QuantPrime. The figure shows an example of the 'Results' page. Primer pairs successfully identified for the examined transcripts are presented. The following information is provided: the sequences (5' to 3') of the forward and reverse primers; the amplicon size (in bp); whether at least one primer spans an exon-exon junction ('Yes' in all cases in the example shown); the rank score (as calculated by Primer3); and the color code of the specificity rank given to the primer pair (see text for details). When clicking the primer pairs, more details are shown (see Figure 3).