Table 3.
Experimental results of primer pairs designed with QuantPrime
| Experiment | Predicted gDNA-safe | Quality control passed1 | Quality control passed1 for detectable transcripts2 |
| A. thaliana | 113/128 (88.3%) | 117/128 (91.4%) | 117/122 (95.9%) |
| C. reinhardtii | 24/33 (72.7%) | 28/33 (84.8%) | 28/29 (96.6%) |
| H. vulgare3 | - | 27/30 (90.0%) | 27/28 (96.4%) |
| 137/161 (85.1%) | 172/191 (90.1%) | 172/179 (96.1%) | |
The primer pairs were designed for wet-lab quantification experiments. The number of primer pairs passing strict quality control checks (melting curve analysis, agarose gel separation and efficiency check) are reported in the table.
1 Melting curve analysis, gel analysis and efficiency check (E ≥ 1.8) passed. 2 Undetectable transcripts (Ct > 40) removed from the statistics. 3 TIGR Transcript Assembly annotation used, no genomic sequences available.