Table 1.
Statistics of Solea senegalensis cDNA libraries
| Tissue (library) | Recombination efficiency a | Titre (pfu/ml) b | Average insert (kb) | % Representation in master library |
| Larvae/Juvenile | ||||
| Premetamorphosis | 97.0 | 4.2 × 109 | 1.0 | 1.27 |
| Metamorphosis and postmetamorphosis | 97.0 | 4.0 × 109 | 0.9 | 1.93 |
| Abnormal stages c | 97.0 | 2.5 × 109 | 1.0 | 2.38 |
| Undifferentiated gonads d | 98.4 | 1.8 × 108 | 1.4 | 46.10 |
| Adult | ||||
| Brain (including pituitary gland) | 97.3 | 3.0 × 109 | 1.2 | 5.35 |
| Stomach | 97.0 | 6.7 × 109 | 1.2 | 0.97 |
| Intestine | 97.0 | 4.1 × 109 | 1.0 | 1.88 |
| Liver | 97.0 | 6.6 × 109 | 1.1 | 1.17 |
| Ovary e | 98.2 | 2.0 × 108 | 1.0 | 32.68 |
| Testis f | 97.0 | 0.5 × 109 | 1.7 | 6.26 |
aThe recombination efficiency represents the percentage of clones containing inserts; bTitre after one round of amplification; cLarvae showing developmental alterations: albinism, malpigmentation, osteological malformations, and incomplete eye migration; dThree different primary libraries were pooled before amplification; eMixed tissue from ovaries at pre-vitellogenesis, vitellogenesis and maturation stages; fMixed tissue from testes at mid and late spermatogenesis, and at functional maturation stage (i.e., spermiating).