Table 2.
Largest EST clusters (with ≥ 30 ESTs) in the normalized multi-tissue cDNA library of Senegalese sole
| GenBank hit acc. no a | Gene identification (species) of top BLAST hit a | BLAST E-value a |
Length (% identity) b |
No. ESTs |
| ABQ58114 | Vitellogenin Ab (Hippoglossus hippoglossus) | 1E-109 | 185 (78%), | 150 |
| CAC45059 | Type I keratin S8 (Oncorhynchus mykiss) | 1E-137 | 299 (83%) | 136 |
| ABQ58113 | Vitellogenin Aa (Hippoglossus hippoglossus) | 0 | 1293 (73%) | 112 |
| CAH59609 | Apolipoprotein AI precursor (Platichthys flesus) | 2E-70 | 260 (52%) | 98 |
| P68140 | Actin, alpha skeletal muscle A (Takifugu rubripes) | 0 | 377 (100%) | 94 |
| BAB12571 | Myosin heavy chain (Pennahia argentata) | 0 | 779 (95%) | 82 |
| AAY21007 | Zona pellucida protein Bb (Sparus aurata) | 3E-139 | 353 (77%) | 78 |
| BAA85157 | Elongation factor 1 alpha (Seriola quinqueradiata) | 0 | 448 (95%) | 64 |
| AAY21009 | Zona pellucida protein Ba (Sparus aurata) | 5E-164 | 333 (68%) | 54 |
| NP_956752 | Eukaryotic translation elongation factor 2, like (Danio rerio) | 0 | 738 (86%) | 52 |
| ABN80443 | Creatine kinase 1 (Poecilia reticulata) | 0 | 359 (94%) | 47 |
| P61155 | 40S ribosomal protein S19 (Pagrus major) | 8E-64 | 121 (95%) | 46 |
| NP_571231 | Keratin 5 (Danio rerio) | 2E-138 | 270 (75%) | 44 |
| P84335 | Tropomyosin (Pennahia argentata) | 6E-139 | 279 (98%) | 44 |
| AAO43607 | Serum lectin isoform 3 precursor (Salmo salar) | 7E-17 | 45 (34%) | 39 |
| CAA63709 | Chorion protein (Sparus aurata) | 2E-149 | 283 (63%) | 37 |
| ABB17040 | Heat shock cognate 70 (Fundulus heteroclitus) | 3E-100 | 198 (96%) | 32 |
| BAA95131 | Myosin light chain 2 (Pennahia argentata) | 2E-87 | 162 (98%) | 30 |
aMost significant BLASTX hit is reported; bExtent of BLASTX hit aligned region (in amino acids), and percent identity over the aligned region.