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Results from assessment of the viability of primary culture hepatocytes by use of the MTT assay. (a), (b), (c), mechanical separation and cultured in L-15, M199, or DMEM, respectively; (d), (e), (f), two-step perfusion and cultured in L-15, M199, or DMEM, respectively; (g), (h), (i), pancreatin digestion and cultured in L-15, M199, or DMEM, respectively. Each point is the mean from six experiments
