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. Author manuscript; available in PMC: 2008 Dec 30.
Published in final edited form as: Nat Immunol. 2008 May 11;9(6):613–622. doi: 10.1038/ni.1612

Figure 6.

Figure 6

IL-7R and BCR signaling repress Rag transcription through the PI(3)K-Akt signaling axis. (a) GFP expression in B220+IgM+IgD− primary immature B cells from Rag1-GFP-heterozygous mice, cultured for 24 h with Akt or PI(3)K inhibitors or DMSO vehicle alone. (b) GFP expression in large B220+CD43+IgM− bone marrow B cells from Rag1-GFP-heterozygous mice, cultured for 4 d in the presence of IL-7 (2 ng/ml), then treated for an additional 2 d with Akt or PI(3)K inhibitors or DMSO vehicle alone (while still in the presence of IL-7) and then collected and labeled with antibodies to distinguish developing B cell subsets. Data are representative of two independent experiments.