TABLE 5.
Relative expression ratios between FKS genes
| Strain | Genotypec
|
Expression ratio
|
||
|---|---|---|---|---|
| FKS1-HS1 | FKS1-HS2 | FKS1/FKS2 | FKS1/FKS3 | |
| 5314 | WT | WT | 3.33 | 20.00 |
| A15a | S645S/P | WT | 1.41 | 7.14 |
| A15-10a | S645P | WT | 1.15 | 3.57 |
| 36082 | WT | WT | 2.56 | 33.33 |
| 90028 | WT | WT | 2.75 | 25.00 |
| 70b | WT | WT | 3.04 | 27.14 |
| S1b | S645S/P | WT | 1.28 | 12.56 |
| S36b | S645P | WT | 0.74 | 2.17 |
| 119 | F641L | WT | 1.46 | 8.33 |
| 177 | F641S | WT | 0.38 | 4.76 |
| 205 | S645P | WT | 0.97 | 3.85 |
| 89 | S645Y | WT | 0.53 | 4.76 |
| 85 | S645F | WT | 1.47 | 12.50 |
| 149 | D648Y | WT | 2.13 | 25.00 |
| 122 | P649H | WT | 3.92 | 25.00 |
| 121 | WT | R1361H | 1.29 | 9.09 |
| 194 | S645F | R1361R/H | 0.49 | 4.35 |
| 90 | WT | R1361R/H | 1.79 | 33.33 |
Spontaneous mutants selected using ANF and SC5314 as the paternal strain.
Spontaneous mutants selected using CSF and strain 70 as the paternal strain (3).
WT, wild type.