FIG. 2.
Transport of GS-9148 and adefovir by hOATs. (A) hOAT1. [3H]adefovir and [14C]GS-9148 at a 10 μM concentration were incubated with CHOhOAT1 cells and CHOpIRES cells (control), and the cellular uptake of tested nucleotides was determined at various time points in the absence and presence of 500 μM probenecid (PBC). (B) hOAT3. Experiments were conducted under the same conditions except with longer incubation times and using BHKhOAT3 cells and BHKpIRES cells (control). The data for both transporters are presented as means ± standard deviations from two independent experiments performed in duplicate. At all analyzed time points, the accumulation of both drugs in the transporter-expressing cells was significantly different from that in the control cells and the transporter-expressing cells treated with PBC (P < 0.005 for hOAT1 and P < 0.01 for hOAT3 using a two-tailed paired t test).