FIG. 5.
In vivo efficacies of indenoisoquinolines. Mice were dosed with indenosioquinoline compound 12 (open circles), compound 20 (filled circles), or compound 23 (filled squares) at 50 mg/kg and challenged with 5 × 105 T. brucei. Berenil (open squares) served as a positive drug control. Untreated animals (filled diamonds) and mice dosed with compounds 20 or 23 had detectable parasitemia 2 days after infection and succumbed by day 4. Indenoisoquinoline compound 12 delayed the appearance of parasites until 4 days postinfection and increased survival to 6 days. The lower limit of detection of parasitemia was log10 3 (103 cells/ml).