FIG. 1.

Effect of metabolic inhibitors on the candidacidal activities of HBD-1 to HBD-3 and Phd1 to Phd3 against C. albicans. Cells pretreated with 50 μM CCCP or 5 mM sodium azide for 2 h at 30°C were further incubated with peptides at the MFC in PB for 2 h at 30°C. Suitably diluted aliquots were plated onto YEPD agar plates, which were incubated for 24 h. The colonies formed were counted, and the percent killing of C. albicans cells was determined. Shaded bars and dark bars represent peptide activities in the presence of 50 μM CCCP and 5 mM sodium azide, respectively. The values represent averages of results from three independent experiments done with duplicate samples, and variations were 3%.