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. 2008 Nov 7;75(1):45–53. doi: 10.1128/AEM.01723-08

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FIG. 1. — Western blot analyses of putative strain B728a AHL acylases expressed in E. coli and strain B728a. (A) Insoluble (i) and soluble (s) fractions collected from induced E. coli harboring plasmids pET30 (lanes 1 and 2), pET30-HacA (lanes 3 and 4), pET30-HacB (lanes 5 and 6), and pET30-Psyr_3871 (lanes 7 and 8). (B) Insoluble (i) and soluble (s) fractions collected from strain B728a harboring plasmids p519nGFP (lanes 1 and 2), p519-HacA (lanes 3 and 4), p519-HacB (lanes 5 and 6), and p519-Psyr_3871 (lanes 7 and 8).