TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| E. coli strains | ||
| Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen, Carlsbad, CA |
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3) | Novagen, Madison, WI |
| MT102 | araD139 Δ(ara-leu)7697 Δlac thi hsdR, MC1000 derivative | 73 |
| P. syringae strains | ||
| B728a | Rfr, wild type, bean pathogen | 39 |
| BHSL | Rfr Kmr, ΔahlI::MudI1734, strain B728a derivative | 30 |
| HacA− | Rfr Tetr, ΔhacA::Tet, strain B728a derivative | This study |
| HacB− | Rfr Tetr, ΔhacB::Tet, strain B728a derivative | This study |
| HacA−B− | Rfr Tetr Gmr, ΔhacA::Gm, ΔhacB::Tet, strain B728a derivative | This study |
| Plasmids | ||
| pCR2.1-TOPO | Apr Kmr, cloning vector | Invitrogen |
| pENTR/D-TOPO | Kmr, entry vector for Gateway cloning system | Invitrogen |
| pENTR/D-MCS-Kan | Kmr Gmr, entry vector for Gateway cloning system | T. K. Powell, G. F. J. Dulla |
| pET30 | Kmr, inducible expression vector | Novagen |
| pET30-HacA | Kmr, pET30 containing hacA ORF with C-terminal six-His tag | This study |
| pET30-HacB | Kmr, pET30 containing hacB ORF with C-terminal six-His tag | This study |
| pET30-Psyr_3871 | Kmr, pET30 containing Psyr_3871 ORF with C-terminal six-His tag | This study |
| p519nGFP | Kmr, broad-host-range expression vector | 42 |
| p519-HacA | Kmr, p519 containing hacA ORF with C-terminal six-His tag | This study |
| p519-HacB | Kmr, p519 containing hacB ORF with C-terminal six-His tag | This study |
| P519-Psyr_3871 | Kmr, p519 containing Psyr_3871 ORF with C-terminal six-His tag | This study |
| pLVC/D | Apr Tetr, Gateway destination vector strain B728a suicide plasmid | 40 |
| pBQ9 | Spcr, pPROBE-OT derivative harboring P. syringae ahlI promoter upstream of GFP | 52 |
| JB524 | Tetr, pME6031 derivative harboring V. fischeri luxR,PluxI-gfpmut3* (stable GFP) | 73 |
a
Ap, ampicillin; Gm, gentamicin; Km, kanamycin; Rf, rifampin; Spc, spectinomycin; Tet, tetracycline.