TABLE 2.
Characteristics of E. coli strains used for in vivo experimentsa
| Strain | Serotype | EcoR group | MLST results | Serum testb | Virulence gene profile
|
No. of VAGs | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Adhesins
|
Iron acquisition
|
Protectin
|
Invasins, toxins, and others
|
||||||||||||||||||||||||||||||
| hra | iha | mat | papAH | papC | papEF | papG | tsh | chuA | fyuA | ireA | iroN | irp2 | iucD | iutA | sit (chromosomal) | sit (episomal) | iss | kpsMT | neuC | traT | EAST-1 | vat | gimB | ibeA | tia | cvi/cva | malX | ||||||
| Outbreak APEC strain | |||||||||||||||||||||||||||||||||
| IMT5155 | O2:K1:H5 | B2 | ST140/STC95 | R | − | − | + | − | − | − | − | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | + | + | + | − | + | + | 22 |
| Nonpathogenic control strain | |||||||||||||||||||||||||||||||||
| IMT11327 | Ont:H16 | B1 | ST295/none | R | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | 2 |
| Nonoutbreak strains (Afecal/Aenviron) | |||||||||||||||||||||||||||||||||
| IMT6483 | Ont:H4 | B2 | New/none | R | − | − | + | − | − | − | − | − | + | + | − | − | + | − | − | + | − | + | + | − | + | − | + | + | − | − | + | + | 14 |
| IMT6487 | O8:H51 | B2 | New/none | R | + | − | + | − | − | − | − | − | + | + | − | − | + | + | + | − | − | − | − | − | + | + | + | − | + | − | + | + | 15 |
| IMT10666c | O59:H21 | B1 | ST58/STC155 | R | − | − | + | − | − | − | − | + | − | − | − | + | − | + | + | − | + | + | − | − | + | − | − | + | − | − | − | − | 11 |
| IMT10676c | O8:Hnt | B1 | New/none | R | − | − | + | − | − | − | − | − | − | − | − | + | − | + | + | − | + | + | − | − | + | − | − | − | − | − | + | − | 10 |
| IMT10740c | O2:H6 | B2 | New/none | R | − | − | + | − | − | − | − | + | + | + | − | + | + | + | + | + | + | + | + | − | + | − | + | − | + | − | + | + | 19 |
| IMT12205 | O43:H2 | B1 | ST920/none | S | + | − | + | − | − | − | − | − | − | + | − | − | − | + | + | − | − | − | − | − | + | + | + | − | + | − | + | + | 13 |
| IMT12226 | O77:H18 | D | NTd | R | − | − | + | + | + | + | + | + | + | − | + | + | − | + | + | + | − | + | − | − | + | + | − | − | − | + | + | − | 19 |
| IMT13060 | Ont:H6 | B2 | ST115/none | R | − | − | + | − | + | − | - | + | + | − | + | + | − | + | + | + | − | + | − | − | + | + | − | − | − | + | + | + | 17 |
| IMT14782 | Ont:NM | B2 | ST69/STC69 | R | + | + | + | − | + | − | + | − | + | + | + | + | + | + | + | + | − | + | − | − | + | + | − | − | − | + | + | − | 21 |
| IMT15146 | O1:H7 | B2 | ST95/STC95 | R | − | + | + | + | + | + | + | − | + | + | + | + | + | + | + | + | + | + | + | + | + | − | + | + | − | + | + | + | 26 |
| IMT15147 | O21:H4 | B2 | ST429/none | R | − | + | + | − | − | − | − | − | + | + | − | + | + | + | + | + | + | + | + | + | + | − | + | − | + | + | + | 20 | |
| IMT15151 | Ont:H31 | D | ST420/none | R | + | − | + | − | − | − | − | + | + | + | − | − | + | + | + | + | + | + | + | + | + | + | − | − | + | − | + | − | 19 |
| IMT15152 | Ont:H4 | B2 | ST428/none | R | − | − | + | − | − | − | − | − | + | + | − | + | + | − | − | + | + | + | + | + | + | − | + | − | + | − | + | + | 17 |
| IMT15153 | O21:H4 | B2 | ST429/none | R | − | − | + | − | − | − | − | − | + | − | + | + | + | − | − | + | + | + | + | + | + | + | + | − | + | − | + | + | 18 |
a
Results for genes for which all strains were either negative (afa/draB, bfp, bmaE, gafD, nfaE, sfa/foc, sfaS, focG, cnf1/2, hlyA, sat, stx1/2, pic, escV, and pks) or positive (fimC and ompA) are not shown, but they were used for calculation of the total number of VAGs per strain. +, gene present; −, gene not present.
b
R, serum resistant; S, serum sensitive.
c
Aenviron strain.
d
NT, not tested.