FIG. 7.

Phosphorylation of the Hog1-like MAPK in A. brassicicola after exposure to fungicides. Germinated conidia from the WT strain (Abra43), the derived spontaneous mutant (Abra43M), and the disrupted mutant (nik1Δ3) were grown for 24 h in PDB and exposed to 100 mg/liter iprodione (Ip) or fludioxonil (Fl) or to 1 mg/liter ambruticin (Am) for 10 min. Control (Co) cultures were not supplemented with fungicides. Total protein extracts, prepared from the harvested mycelia, were analyzed by SDS-PAGE and blotting with either anti-Hog1 C-terminus antibody or anti-dually phosphorylated p38 antibody.