FIG. 8.

Effects of fungicides on the growth and phosphorylation of Os2 MAPK of N. crassa isolates. (A) The growth of WT and mutant N. crassa isolates was monitored after 24 h of incubation at 25°C on agar-solidified Vogel's medium supplemented with 25 mg/liter iprodione (Ip25) or fludioxonil (Fl25) or 0.1, 1, or 10 mg/liter ambruticin (Am 0.1, Am 1, or Am 10, respectively) or not supplemented (control [Co]). Three typical growth inhibition patterns were obtained (upper panel, isolates FGSC988 and FGSC16195; middle panel, isolates FGSC2432 and FGSC824; lower panel, isolates FGSC4494, FGSC4576, FGSC13363, and FGSC1509). Names of isolates that were selected for this picture are indicated in bold letters. (B) Phosphorylation of Os2 MAPK in the different N. crassa isolates exposed to fludioxonil (Fl), ambruticin (Am), or iprodione (Ip) or not exposed (Co) was assessed as described in the legend to Fig. 7. Three typical phosphorylation patterns were obtained: one with isolates FGSC988 and FGSC16195; one with isolate FGSC2432; and one with isolates FGSC4494, FGSC4576, and FGSC13363. The phosphorylation pattern of isolate FGSC824 was not studied, and no signal was obtained with proteins from the Os2-null mutant FGSC1509.