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Phylogenetic tree of LipEH166 and closely related proteins. Phylogenetic analysis was performed using Megalign in the Lasergene program (version 6.0; DNAStar Inc.). Amino acid sequences were aligned by Clustal V. Except for LipEH166 and related proteins, the protein sequences for families of bacterial lipolytic enzymes previously classified by Arpigny and Jaeger (1) and for the LipG family (11) were retrieved from GenBank (http://www.ncbi.nlm.nih.gov).
