TABLE 1.

Effects of several group-specific inhibitors on purified CphE_al from P. alcaligenes strain DIP1

Inhibitor specificity	Inhibitor	Low concn-high concn (mM)	% Inhibition by HPLC analysisb
			Low inhibitor concn	High inhibitor concn
Thiol proteases	Leupeptin	0.001-0.01	10	39
Metalloproteases	EDTA	30-60	65c	75c
Serine proteases	Pefablocd	8-80	91	100
Serine proteases	PMSF	1-10	0	57
Tryptophan residues	N-bromosuccinimide	1-5	100	100
	Controla		0	0

^aControl without inhibitor.

^bThe values for inhibition are relative to the control, that is, the activity of CGPase in the absence of an inhibitor.

^cValues are not due to inhibition but due to the formation of precipitates during OPA derivatization prior to HPLC analysis.

^dPefabloc, 4-(2-aminoethyl) benzenesulfonylfluoride·HCl.