TABLE 2.

Comparison of biochemical characteristics of CphE_al from P. alcaligenes strain DIP1 and previously characterized extracellular cyanophycinases

Bacterium (enzyme)	Enzyme properties
	Localization	Molecular size (kDa)	Optimum temp (°C)	Optimum pH range	CGP degradation product(s)	Mechanism	Inhibitorse
Synechocystis sp. strain PCC 6803 (CphB)a	Intracellular	28	35	7-8	β-Asp-Arg	Exopeptidase	Pefabloc, PMSF, 3,4-dichloroisocoumarin
P. anguilliseptica strain BI (CphEPa)b	Extracellular	43	NDf	8-8.5	β-Asp-Arg	Exopeptidase	Pefabloc, PMSF, N-bromosuccinimide
B. megaterium strain BAC19 (CphEBm)c	Extracellular	37	30-42	8-8.5	β-Asp-Arg + (β-Asp-Arg)2	ND	Pefabloc, PMSF, N-bromosuccinimide
P. alcaligenes strain DIP1 (CphEal)d	Extracellular	45	50	7-8.5	β-Asp-Arg	ND	Pefabloc, PMSF, N-bromosuccinimide

^aData are from the work of Richter et al. (35).

^bData are from the work of Obst et al. (28).

^cData are from the work of Obst et al. (29).

^dThis study.

^ePefabloc, 4-(2-aminoethyl) benzenesulfonylfluoride·HCl.

^fND, not determined.